Previously, Zhang's team had adapted bacterial CRISPR systems into gene-editing ... Sequences within each gene's repetitive region—its TIGR arrays—encode an RNA guide that interacts with ...

Previously, Zhang’s team adapted bacterial CRISPR systems into gene editing ... Sequences within each gene’s repetitive region—its TIGR arrays—encode an RNA guide that interacts with ...

When viruses or phages first invade the bacteria, Cas proteins recognize and cut the DNA from viruses or phages into short fragments that will be integrated into the CRISPR array. Once bacteria are ...

but there is a bias about type I systems and type III systems in bacteria, hyperthermophiles, and archaea, respectively. In summary, regulation of CRISPR–cas systems occurs at transcriptional level of ...

In some CRISPR systems, an imprecise match between a spacer and ... including primed acquisition directly would require expanding bacterial arrays to at least two spacers. The acquisition probability ...

32 An expanding array of genetic tools is available for T-cell engineering, including γ-retroviral, lentiviral, and non-viral DNA vectors (eg, the Sleeping Beauty transposon system), RNA-based vectors ...

says Modell. The Johns Hopkins investigators say bacteria have long been known to use CRISPR-Cas systems to chop up phage DNA, break it down and get rid of it. Crucially, CRISPR systems can only ...

Now, Johns Hopkins Medicine scientists say they have shed new light on how bacteria protect themselves from certain phage ...