Current CRISPR guide RNAs are designed based on European reference genomes, leading to false-negative results in cells from ...

A Ph.D. student in biomolecular engineering at the University of California, Santa Cruz, has built a software program ...

Advanced forms of CRISPR allow researchers to rapidly screen diseased versus normal cells, finding targets for gene editing. Scientists used to spend years and "tons of money" breeding mice that ...

CRISPR screen identifies EIF3D as critical regulator of stem cell pluripotency maintenance. Story by Science X staff • 11h. A team of CiRA researchers has uncovered the crucial role of EIF3D—a ...

Li M et al. Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution. Cell Rep . 2018; 24: 489–502. Doench JG.

CRISPR Screen Detects Functional Gene Regulation. A CRISPR-Cas9–based method probes the regulatory roles of noncoding DNA sequences. Abby Olena, PhD. Abby Olena, PhD. As a freelancer for The Scientist ...

A CRISPR-based loss-of-function screen has identified host genes that are needed for SARS-CoV-2 to infect human lung cells, pointing to potential novel treatment targets for COVID-19. SARS-CoV-2 ...

“We used the CRISPR-Cas9 system to perform genome-wide gene-knockout screens for suppressors and enhancers of C9ORF72 DPR toxicity in human cells,” the authors wrote.“We validated hits by ...

CRISPR screen points to spinal repair pathway in zebrafish. By Arlene Weintraub Apr 30, 2021 8:15am. regenerative medicine spinal cord injury ...

“In the CRISPR screen, we used human liver cells and knocked out every gene in the genome—about 19,000 genes—one at a time. We then stimulated the cells with interferon, knowing that this ...